Neuromodulation of Innervated In Vitro Models
Projects focusing on in vitro neuromodulation and microenvironmental modeling have grown in popularity with the introduction of biomaterials to simulate in vivo conditions, electrical stimulation and analysis devices such as microelectrode arrays, and advanced culturing techniques in platforms that allow for axon growth and interaction with other cell types. Since debilitating neurological disorders affect many in the world, it is important that experimentation often seeks to discover regenerative sources or infer deeper knowledge of normal vs disease-state neural mechanisms.
Researchers at ABNEL are currently undergoing projects involving both the central and peripheral nervous systems. Multiple technologies, including ABNEL’s organ-on-chip devices, are being harnessed to modulate neural behaviors and relationships with other cells. For example, targeted optogenetic stimulation is used to employ axonal regenerative capabilities of Schwann cells and selectively activate autonomic nerves to recapitulate cardiac innervation and determine roles in human development. Biomaterials are also being developed to mimic the extracellular matrix and allow for complex, realistic 3D creation of neural tissues
Recent publications:
Tess Torregrosa, Sophie Webster, Chiamaka Aghaizu, Jonathan Soucy, Christopher Bertucci, Leigh Plant, Abigail Koppes, Ryan Koppes. Cryopreservation and functional analysis of cardiac autonomic neurons. July 2020. https://doi.org/10.1016/j.jneumeth.2020.108724
Ventre, Daniel; Puzan, Marissa; Ashbolt, Emily*; Koppes, Abigail. Enhanced total neurite outgrowth and secondary branching in dorsal root ganglion neurons elicited by low intensity pulsed ultrasound. Journal of Neural Engineering. April 2018. DOI: https://doi.org/10.1088/1741-2552/aabeba
Recent review:
Christopher Bertucci, Ryan Koppes, Courtney Dumont, and Abigail Koppes. Neural responses to electrical stimulation in 2D and 3D in vitro environments. July 2019. https://doi.org/10.1016/j.brainresbull.2019.07.016
ABNEL lab members recognized at departmental award ceremony!
Several lab members were recognized today at the chemical engineering department award ceremony. Congratulations everyone! Caroline Mills: Best MS student researcher (Class 2019-2020), undergraduate research award, Adam Bindas: 2020 outstanding seminar award Beth DiBiase: 2020 Nabil Morris award Caroline Ghio: Huntington 100, 2020 college of engineering rising star award, Peak summit award Bridget Eckel: Advanced […]
Caroline successfully defends her thesis
Caroline Mills defended her master’s thesis today titled: Using magnetic and topographical cues to influence the growth of neuron extensions. Congratulations Caroline!